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Image Search Results
Journal: Cell stem cell
Article Title: Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification
doi: 10.1016/j.stem.2018.07.001
Figure Lengend Snippet: (A) Schematic representation of the directed cardiac differentiation protocol in T-GFP mouse ESCs.
Article Snippet:
Techniques:
Journal: Cell stem cell
Article Title: Tbx6 Induces Nascent Mesoderm from Pluripotent Stem Cells and Temporally Controls Cardiac versus Somite Lineage Diversification
doi: 10.1016/j.stem.2018.07.001
Figure Lengend Snippet: KEY RESOURCES TABLE
Article Snippet:
Techniques: Recombinant, CRISPR, Software
Journal: Biology Open
Article Title: Requirement for STAT3 and its target, TFCP2L1, in self-renewal of naïve pluripotent stem cells in vivo and in vitro
doi: 10.1242/bio.059650
Figure Lengend Snippet: STAT3 and TFCP2L1 are required for ICM maintenance during diapause, but STAT3 is not required for ESC derivation or self-renewal. (A,B) IF of NANOG, pY705 and TFCP2L1 in diapause for Stat3 WT/het and null embryos (A) and Tfcp2l1 WT/het and null embryos (B). Scale bars: 20 µm. (C) Number of ICM cells in Stat3 or Tfcp2l1 WT/Het and null diapause embryos. (D) Brightfield images of WT and Stat3 null ESCs. Scale bars: 100 µm. (E) Proliferation based on cell number counts of WT and Stat3 null ESCs cultured in N2B27+2i medium for 5 days (day 4, P =0.14; day 5, P =0.55 by Student's t -test). (F) IF of OCT4 (red) and NANOG (green) in WT and Stat3 null ESCs. Scale bars: 100 µm.
Article Snippet: Tfcp2l1 heterozygous mice were generated from ESCs targeted using CRISPR strategy in
Techniques: Cell Culture